Composite

Part:BBa_K4773011:Design

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-01)


Lac Promoter+RBS+NicA+RBS+NicB+RBS+NicC+Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1421
    Illegal BglII site found at 1662
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Term (BBa_B0015) was chosen because it is a double terminator, which can effectively terminate transcription and protect mRNA from degradation. Different restriction sites were added between each part to facilitate the connection of each part or further manipulation. RBS (BBa_B0034) was chosen because it has a high translation initiation rate and is compatible with most bacterial hosts.Lac Promoter was chosen because it can regulate the expression of downstream genes according to the presence or absence of lactose, thus achieving inducible control.


Source

Lac Promoter: From the lac operon of Escherichia coli. RBS: From the standard biobrick provided by NEB company. NicA, NicB and NicC: From the genomic sequence of Pseudomonas putida S16. Term: From the standard biobrick provided by NEB company.

References